Table 3 Comparative data of RT-qPCR and RNA-Seq for 7 differentially expressed conserved miRNAs
From: Systematic miRNome profiling reveals differential microRNAs in transgenic maize metabolism
miRNA | Comp. | Samples | Ct Rep. 1 | Ct Rep. 2 | Ct Rep. 3 | Read count | Log2FC RT-qPCR | Log2FC RNA-Seq |
|---|---|---|---|---|---|---|---|---|
zma-MIR167c | Bt vs Control | Bt S1 | 26.59 | 26.56 | 26.68 | 103 | 0.58 | 0.56 |
Bt S2 | 26.67 | 26.49 | 26.62 | 304 | ||||
Bt S3 | 26.66 | 26.37 | 26.38 | 230 | ||||
Conv S1 | 27.33 | 27.31 | 27.58 | 199 | ||||
Conv S2 | 27.53 | 27.85 | 27.69 | 132 | ||||
Conv S3 | 27.39 | 26.97 | 26.79 | 170 | ||||
zma-MIR167ej | Bt vs Control | Bt S1 | 26.22 | 26.17 | 26.11 | 325 | 0.11 | 0.55 |
Bt S2 | 25.99 | 25.95 | 25.93 | 515 | ||||
Bt S3 | 26.11 | 26.17 | 26.10 | 428 | ||||
Conv S1 | 26.46 | 26.53 | 26.54 | 336 | ||||
Conv S2 | 26.55 | 26.65 | 26.49 | 293 | ||||
Conv S3 | 26.24 | 26.31 | 26.11 | 380 | ||||
zma-MIR169f | Bt vs Control | Bt S1 | 26.81 | 26.34 | 26.27 | 108 | − 1.05 | − 0.78 |
Bt S2 | 26.35 | 26.87 | 27.07 | 118 | ||||
Bt S3 | 26.43 | 26.13 | 26.73 | 170 | ||||
Conv S1 | 25.11 | 24.88 | 25.09 | 77 | ||||
Conv S2 | 26.61 | 25.63 | 25.76 | 176 | ||||
Conv S3 | 26.80 | 26.84 | 26.62 | 115 | ||||
zma-MIR169Â m | Bt vs Control | Bt S1 | 28.12 | 27.99 | 28.14 | 561 | 0.42 | 0.62 |
Bt S2 | 27.36 | 27.42 | 27.53 | 852 | ||||
Bt S3 | 27.56 | 27.81 | 27.14 | 642 | ||||
Conv S1 | 28.95 | 28.27 | 28.51 | 447 | ||||
Conv S2 | 28.33 | 28.41 | 27.90 | 617 | ||||
Conv S3 | 28.40 | 28.27 | 27.89 | 433 | ||||
zma-MIR399ah | Bt vs Control | Bt S1 | 29.68 | 29.44 | 29.75 | 26 | − 0.29 | − 0.70 |
Bt S2 | 29.43 | 29.36 | 29.14 | 48 | ||||
Bt S3 | 29.22 | 29.34 | 29.12 | 34 | ||||
Conv S1 | 29.41 | 29.09 | 28.90 | 55 | ||||
Conv S2 | 29.69 | 29.19 | 29.62 | 40 | ||||
Conv S3 | 28.98 | 29.99 | 28.99 | 44 | ||||
zma-MIR399eij | RR×Bt vs Control | RR×Bt S1 | 22.15 | 22.09 | 22.12 | 63 | − 1.51 | − 0.64 |
RR×Bt S2 | 22.27 | 22.35 | 22.31 | 91 | ||||
RR×Bt S3 | 22.47 | 22.36 | 22.29 | 107 | ||||
Conv S1 | 20.61 | 20.66 | 20.72 | 116 | ||||
Conv S2 | 21.09 | 21.08 | 21.07 | 107 | ||||
Conv S3 | 20.71 | 20.71 | 20.82 | 165 | ||||
zma-MIR399eij | RR×Bt vs RR | RR×Bt S1 | 22.15 | 22.09 | 22.12 | 63 | 0.00 | − 0.86 |
RR×Bt S2 | 22.27 | 22.35 | 22.31 | 91 | ||||
RR×Bt S3 | 22.47 | 22.36 | 22.29 | 107 | ||||
RR S1 | 23.11 | 23.09 | 23.14 | 146 | ||||
RR S2 | 22.10 | 22.07 | 22.16 | 203 | ||||
RR S3 | 23.39 | 23.46 | 23.41 | 150 | ||||
zma-MIR827 | RR×Bt vs Control | RR×Bt S1 | 27.27 | 26.97 | 26.99 | 479 | − 0.58 | − 0.54 |
RR×Bt S2 | 27.33 | 27.35 | 27.52 | 552 | ||||
RR×Bt S3 | 27.37 | 27.35 | 27.25 | 554 | ||||
Conv S1 | 26.92 | 26.84 | 26.63 | 683 | ||||
Conv S2 | 26.53 | 26.87 | 27.18 | 602 | ||||
Conv S3 | 26.98 | 26.53 | 26.67 | 675 | ||||
zma-MIR827 | RR×Bt vs RR | RR×Bt S1 | 27.27 | 26.97 | 26.99 | 479 | − 1.00 | − 0.50 |
RR×Bt S2 | 27.33 | 27.35 | 27.52 | 552 | ||||
RR×Bt S3 | 27.37 | 27.35 | 27.25 | 554 | ||||
RR S1 | 26.69 | 26.64 | 26.70 | 656 | ||||
RR S2 | 26.80 | 26.81 | 27.00 | 718 | ||||
RR S3 | 26.74 | 26.59 | 26.54 | 751 |