Table 2 Primers used to quantify the transcriptional activity of alkaline phosphatase and phosphate transporter genes in Anabaena sp. FACHB 1299
From: A qPCR method to quantify bioavailable phosphorus using indigenous aquatic species
Primer | Gene accession | Sequence (5′–3′) | Tm (°C) | Product (bp) |
|---|---|---|---|---|
pst1-F | MH184530 | GCCACAGCTCAAGCTCAAAC | 60 | 138 |
pst1-R | CCCACCACCACTACCAATCC | |||
phoA-F | MH184531 | GTGGCTGGAGCAAGAACTTA | 60 | 171 |
phoA-R | CAGCATCTTGAGGGTTGTGT | |||
phoAlike-F1 | MH184532 | TCGGCAGGAATAGTCAAGGT | 60 | 124 |
phoAlike-R1 | AAGTCATCGCCACTGTCGTA | |||
16s-F | 14088448 | AAGCATCGGCTAACTCC | 60 | 199 |
16s-R | TTTCACCGCTACACCAG |