DNA metabarcoding reveals the complex and hidden responses of chironomids to multiple stressors

Beermann, Arne J.; Zizka, Vera M. A.; Elbrecht, Vasco; Baranov, Viktor; Leese, Florian

doi:10.1186/s12302-018-0157-x

Environmental Sciences Europe

Table 1 Summary of (M)ANOVA results for the habitat “streambed”

From: DNA metabarcoding reveals the complex and hidden responses of chironomids to multiple stressors

Streambed	% reads	% habitat reads	N	S	V	N × S	C	N × V	C	S ×TV	C	N × S × V	p
Response variable	% reads	% habitat reads	p value	p value	p value	p value	C	p value	C	p value	C	p value	p
Total read abundance			0.730	< 0.001 (0.32)↑	0.001 (0.18)↑	0.349		0.726		0.369		0.520
Simpson’s diversity			0.193	0.007 (0.12)↑	0.161	0.401		0.159		0.108		0.732
Pielou’s evenness			0.970	0.105	0.227	0.571		0.163		0.437		0.029 (0.08)
OTU richness			0.832	< 0.001 (0.38)↑	0.261	0.833		0.473		0.660		0.968
Community composition (MANOVA, 24 OTUs)	27.9	89.1	0.276	<0.001 (0.87)	0.000 (0.73)	0.796		0.618		0.504		0.552
OTU_1	6.17	19.7	0.453	< 0.001 (0.29)↑	0.034 (0.08)↑	0.641		0.927		0.421		0.859	A
OTU_3	4.51	14.4	0.775	0.908	0.933	0.183		0.309		0.924		0.367	B
OTU_4	2.03	6.5	< 0.001 (0.20) ↑	< 0.001 (0.65)↑	0.111	0.606		0.967		0.033 (0.08)	+ S	0.370	C
OTU_9	1.17	3.7	0.078 (0.05) ↑	< 0.001 (0.54)↑	0.004 (0.14)↑	0.279		0.816		0.317		0.634	A
OTU_2	0.90	2.9	0.934	0.947	0.092 (0.05) ↓	0.222		0.740		0.977		0.479	B
OTU_5	0.75	2.4	0.624	0.463	0.079 (0.05) ↑	0.650		0.518		0.496		0.826	B
OTU_7	0.46	1.5	0.306	0.081 (0.05)↑	0.820	0.997		0.685		0.603		0.248	B
OTU_18	0.40	1.3	0.758	< 0.001 (0.33)↑	0.563	0.372		0.532		0.413		0.218	D
OTU_466	0.33	1.1	0.730	0.097	0.252	0.726		0.752		0.129		0.864	B
OTU_262	0.30	1.0	0.428	< 0.001 (0.36)↑	0.095 (0.05)↑	0.999		0.188		0.066 (0.06)	+ S	0.149	D
OTU_17	0.29	0.9	0.394	0.001 (0.18)↑	0.096 (0.05)↑	0.407		0.770		0.954		0.814	D
OTU_11	0.28	0.9	0.248	0.840	0.842	0.285		0.691		0.070 (0.06)	− S	0.397	B
OTU_16	0.27	0.9	0.066 (0.06) ↓	0.002 (0.16)↓	0.330	0.671		0.181		0.479		0.285	E
OTU_21	0.26	0.8	0.107	0.005 (0.13)↑	0.223	0.211		0.409		0.440		0.209	D
OTU_6	2.18	7.0	0.252	0.065 (0.06)↑	0.003 (0.14)↑	0.014 (0.10)	+ S	0.021 (0.09)	+ S	0.653		0.285	F
OTU_15	1.39	4.5	0.998	0.765	0.005 (0.13)↑	0.285		0.589		0.303		0.412	G
OTU_12	1.15	3.7	0.211	0.142	< 0.001 (0.29)↑	0.921		0.957		0.234		0.489	G
OTU_8	0.97	3.1	0.483	0.976	0.002 (0.16)↑	0.627		0.569		0.705		0.533	G
OTU_14	0.96	3.1	0.953	0.065 (0.06) ↑	0.068 (0.06)↑	0.606		0.499		0.531		0.525	B
OTU_23	0.77	2.5	0.391	0.028 (0.08)↑	< 0.001 (0.21)↑	0.534		0.795		0.261		0.472	A
OTU_28	0.87	2.8	0.727	< 0.001 (0.51)↑	< 0.000 (0.25)↑	0.860		0.961		0.819		0.176	A
OTU_31	0.57	1.8	0.686	0.987	0.195	0.543		0.672		0.601		0.727	B
OTU_19	0.47	1.5	0.770	0.003 (0.15)↑	0.126	0.931		0.746		0.485		0.235	D
OTU_25	0.44	1.4	0.493	0.005 (0.13)↑	0.027 (0.08)↑	0.302		0.045 (0.07)	+ S	0.320		0.982	H

Significant p values (< 0.05) are printed in bold. Effect sizes (partial η², range 0–1) are presented in brackets for p values < 0.1. Arrows indicate increase (↑) and decrease (↓) of abundance under the stressor condition compared to the non-stressor condition. Multivariate p values are for the Pillai’s Trace statistic. Interactions of combined stressors (C) were positive synergistic (+ S) or negative synergistic (− S). OTUs analyzed for both microhabitats are indicated by a bold underline, while OTUs being more exclusive to the streambed are indicated by a underline. N salinity, S fine sediment, V velocity, P response pattern

Back to article page