Fig. 2

Developmental delays at 24 hpf (in %) for samples from the main sampling sites throughout all sampling events. a, d, g, l–n show results from the River Nidda, b, e, h, k from River Horloff and c, f, i, l from River Usa. Asterisks mark significant differences to control treatments (no developmental delays detected in controls; data not shown); Likelihood ratio χ2, Fisher’s exact test (p < 0.05), correction with sequential Bonferroni–Holm method p < α. Photograph o shows a control embryo at 24 hpf with detached tail, normally developed eyes and somites. Photograph p depicts a clearly retarded embryo lacking tail detachment and showing delay in eye and somite development