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Table 2 Overview of the in vitro bioassays, endpoints, reference compounds, EC values and literature source of the methods [EC10: Concentration causing 10% effect relative to the maximum triggered by a positive control (Eq. 3); ECIR1.5: Concentration causing an induction ratio of 1.5 (Eq. 4); ECSR0.2: Concentration causing a suppression ratio of 0.2 in the presence of the agonist (Eq. 5)]

From: Combining in vitro reporter gene bioassays with chemical analysis to assess changes in the water quality along the Ammer River, Southwestern Germany

Assays

Mode of action

Reference compound

EC value

Reference method

ERα-GeneBLAzer

Estrogenicity

17β-Estradiol (E2)

EC10

[15]

GR-GeneBLAzer

Glucocorticogenic activity

Dexamethasone

EC10

[27, 32]

AR-GeneBLAzer

Androgenicity

Metribolone (R1881)

EC10

[15, 33]

Anti AR-GeneBLAzer

Anti androgenicity

Cyproterone acetate (antagonist)

Metribolone R1881 (agonist)

ECSR0.2

[15, 33]

PR-GeneBLAzer

Progestagenic activity

Promegestone

EC10

[15, 34]

Anti PR-GeneBLAzer

Anti progestagenic activity

Mifepristone RU-486 (antagonist)

Promegestone (agonist)

ECSR0.2

[15, 34]

AREc32

Oxidative stress response (adaptive stress response)

t-Butyl-hydroquinone (tBHQ)

ECIR1.5

[28, 29]

PPARγ-GeneBLAzer

Peroxisome proliferation activation

Rosiglitazone

EC10

[30]

AhR-CALUX

Aryl hydrocarbon receptor induction

2,3,7,8-Tetrachlorodibenzodioxin (TCDD)

EC10

[31] with modifications of [16]