Table 4 Description of reference genes and transgenes used for quantification of transcripts and their primer sequences
From: Levels of DNA methylation and transcript accumulation in leaves of transgenic maize varieties
Primer name | Gene product | Genbank accession no. | Primer sequence |
|---|---|---|---|
MEP | Membrane protein PB1A10.07c | GRMZM2G018103_T01 | F-GTACTCGGCAATGCTCTTGA |
P-AACTTCGGTTGGTGAGAGCGGAAA | |||
R-CAATCCTGACCCAGACAGATG | |||
LUG | Leunig | GRMZM2G425377_T01 | F-GGGACATAAGGGAGAAGAACAC |
P-TTCCCTGTAGCACTGGATGATGCC | |||
R-TCATGGCTTACTGAGGCAAC | |||
Cry1A.105 | Cry1A.105 protein | FV532179/FB707509 | F-GACGTGGAGGAACAGAACAA |
P-TTGTGCCTGAGTGGGAAGCTGAA | |||
R-CCTCTACCTGGACAGACTCTAA | |||
Cry2Ab2 | Cry2Ab2 protein | FV532179/AR260587 | F-GCGACTACCTGAAGAACTACAC |
P-CAACACCTACCAGTCGGCCTTCAA | |||
R-TGTCGTGAAGCCTCGTATTG |