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TableĀ 4 Description of reference genes and transgenes used for quantification of transcripts and their primer sequences

From: Levels of DNA methylation and transcript accumulation in leaves of transgenic maize varieties

Primer name Gene product Genbank accession no. Primer sequence
MEP Membrane protein PB1A10.07c GRMZM2G018103_T01 F-GTACTCGGCAATGCTCTTGA
P-AACTTCGGTTGGTGAGAGCGGAAA
R-CAATCCTGACCCAGACAGATG
LUG Leunig GRMZM2G425377_T01 F-GGGACATAAGGGAGAAGAACAC
P-TTCCCTGTAGCACTGGATGATGCC
R-TCATGGCTTACTGAGGCAAC
Cry1A.105 Cry1A.105 protein FV532179/FB707509 F-GACGTGGAGGAACAGAACAA
P-TTGTGCCTGAGTGGGAAGCTGAA
R-CCTCTACCTGGACAGACTCTAA
Cry2Ab2 Cry2Ab2 protein FV532179/AR260587 F-GCGACTACCTGAAGAACTACAC
P-CAACACCTACCAGTCGGCCTTCAA
R-TGTCGTGAAGCCTCGTATTG
  1. F forward primer, R reverse primer, P probe