Table 2 Bisulfite primers used to amplify four DNA regions after bisulfite conversion
From: Levels of DNA methylation and transcript accumulation in leaves of transgenic maize varieties
Primer name | Sequence | GM region | Ta (°C) |
|---|---|---|---|
BS35S-F | 5′-TTATTAAAAGGATAGTAGAAAAGG-3′ | P-e35S | 51 |
BS35S-R | 5′-AAAACCTCCCTTAAATCTTATAAT-3′ | ||
BScry1A.105-F | 5′-ATTATTTGGGGTATTTTTGGTTTTTT-3′ | cry1A.105 | 55 |
BScry1A.105-R | 5′-AAACCCCACCTCTAACCAAACA-3′ | ||
BSFMV-F | 5′-GTTTGTGGGGATTAGATAAAAAA-3′ | P-FMV | 50 |
BSFMV-R | 5′-CACACAAAAAAACATTCAAAAAAA-3′ | ||
BScry2Ab2-F | 5′-TTTATGTTTTGTTTGTTGTTAGAGTGA-3′ | cry2Ab2 | 51 |
BScry2Ab2-R | 5′-ACCAAAAATATTCCTAATAAAATAAT-3′ |