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Table 2 Bisulfite primers used to amplify four DNA regions after bisulfite conversion

From: Levels of DNA methylation and transcript accumulation in leaves of transgenic maize varieties

Primer name Sequence GM region Ta (°C)
BS35S-F 5′-TTATTAAAAGGATAGTAGAAAAGG-3′ P-e35S 51
BS35S-R 5′-AAAACCTCCCTTAAATCTTATAAT-3′
BScry1A.105-F 5′-ATTATTTGGGGTATTTTTGGTTTTTT-3′ cry1A.105 55
BScry1A.105-R 5′-AAACCCCACCTCTAACCAAACA-3′
BSFMV-F 5′-GTTTGTGGGGATTAGATAAAAAA-3′ P-FMV 50
BSFMV-R 5′-CACACAAAAAAACATTCAAAAAAA-3′
BScry2Ab2-F 5′-TTTATGTTTTGTTTGTTGTTAGAGTGA-3′ cry2Ab2 51
BScry2Ab2-R 5′-ACCAAAAATATTCCTAATAAAATAAT-3′
  1. Ta annealing temperature, F forward primer, R reverse primer