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Table 9 Effect concentrations (EC10, EC50 with 95 % confidence interval) and NOEC, (mg/kg soil dry mass) for biomass production of B. napus and P. vulgaris of florfenicol spiked via differently treated manure

From: Results of extended plant tests using more realistic exposure scenarios for improving environmental risk assessment of veterinary pharmaceuticals

Manure Species/endpoint Standard test (no manure) Fresh spiked 1/2 max incubation time Max. incubation time
  B. napus     
Cattle NOEC <0.06 <0.2 5.60 16.7
EC10 0.05 0.07 12.1 27.0
(0.01–0.09) (n.d.) (n.d.) (n.d.)
F   1.4 242 540
EC50 0.25 0.2 20.8 68.0
(0.16–0.38) (n.d.) (n.d.) (n.d.)
F   0.8 83 272
Valid pig   Yes Yes Yes Yes
NOEC <0.06 <0.2 16.7 ≥16.7
EC10 0.05 0.08 14.1* 14.3
(0.01–0.09) (0.07–0.09) (14.0–14.2) (n.d.)
F   1.6 282 286
EC50 0.25 0.21 22.5* 27.8
(0.16–0.38) (0.21–0.22) (22.3–22.7) (n.d.)
Factor   0.84 90 111
Valid   Yes No (67 % emerged, 60 % survived) Yes No (80 % emerged)
  P. vulgaris     
Cattle NOEC 0.56 <0.2 5.60 5.60
EC10 0.76 0.08 11.8* 8.14*
(0.36–1.12) (0.03–0.16) (9.14–14.2) (5.86–10.3)
F   0.1 16 11
EC50 2.59 1.18 28.7* 69.7*
(2.02–3.36) (0.81–1.67) (25.6–32.3) (58.3–88.4)
F   0.5 11 27
Valid pig   Yes Yes Yes Yes
NOEC 0.56 <0.2 16.7 16.7
EC10 0.76 0.07 11.8 28.2*
(0.36–1.12) (0.00–0.27) (n.d.) (24.2–32.2)
F   0.1 16 37
EC50 2.59 1.0 27.0 41.2*
(2.02–3.36) (0.26–2.77) (n.d.) (39.1–42.6)
F   0.4 10 16
Valid   Yes No (80 % emerged) Yes Yes
  1. Concentration range (both species): 0.06–5.0 mg/kg soil (standard tests), 0.2–16.7 mg/kg soil (fresh spiked), 0.62–50.0 mg/kg soil (other tests). Results evaluated as  % inhibition compared to the standard control (in standard tests) or the manure control (in extended tests)
  2. F factor of difference, ECx (manure spiked test)/ECx (standard test)
  3. * Significantly greater than value from standard test (based on overlapping 95 % confidence intervals)