Results of extended plant tests using more realistic exposure scenarios for improving environmental risk assessment of veterinary pharmaceuticals

Environmental Sciences Europe

Table 3 Parameters measured in manure at different stages and methods applied

Parameter	Method	Determined during sampling (1), end of storage (2), end of acclimation (3)
pH	Direct measurement in liquid manure	1, 2, 3
Temperature	Direct measurement in liquid manure	1, 2, 3
Redox potential	Direct measurement in liquid manure	1, 2, 3
Dry matter content	Drying of subsamples of 10-g fresh mass at 105 °C overnight in a drying chamber. Quantification by weighting	2, 3
Microbial activity	For testing the microbial activity several suggestions exist. In this case it was mineralisation of ¹⁴C-labeled glucose under anaerobic conditions	3
NH₄-N content	Ammonium-N, purge and trap-method. Quantification by titration [10]	3
Nonvolatile N content	Analysis of dried manure by determination with element analyser after combustion	3
Total N content	Calculation by addition of NH₄–N content and nonvolatile N content	3
Organic C content	Analysis of dried manure by determination with element analyser after combustion	3
Total P content	Extraction of trace elements soluble in aqua regia [11] and phosphor quantification by ICP-OES with a matrix adjusted calibration [12]	3
Total Cu content	Extraction of trace elements soluble in aqua regia [10] and phosphor quantification by ICP-OES with a matrix adjusted calibration [12]. Copper quantification by ICP-OES with a matrix adjusted calibration	3