Table 3 Organism-level effects and assessed endpoints, according to the research area of studies and the investigated sample matrices
From: The value of zebrafish as an integrative model in effect-directed analysis - a review
Organism-level effect | Endpoint | Research area | Matrix and reference |
|---|---|---|---|
Acute toxicity | Lethality and acute toxicity (respiratory rate, heart rate, movement) endpoints | Environmental toxicology | Sediment extracts [50], industrial effluents [33], rubber tyre leachates [32], oil sand process waters [57] |
Natural products | Cyanobacteria and algae extracts [47], seaweed hydrolysates [48] | ||
Acute and developmental toxicity | Lethal and morphological endpoints | Environmental toxicology | Sediment extracts [51], soil extracts [53], microalgae extracts [54], cyanobacteria extracts [55], river pore water extract [58] |
Natural products | Bacteria extracts [46] | ||
Developmental toxicity | Phenotypic endpoints | Natural products | Plant extracts [41] |
Anti- or pro-angiogenesis | ISV formation and/or function in wild-type or fli1:EGFP zebrafish | Natural products | |
Anti-angiogenesis and anti-inflammatory | ISV outgrowth in fli1:EGFP, leukocyte migration after tail transection | Natural products | Plant extracts [40] |
Glucose uptake | Uptake of fluorescein-tagged glucose bioprobe | Natural products | Plant extracts [45] |
Lipid storage modulation | Uptake and metabolism of fluorescent fatty acid | Natural products | Heterofibrin molecules from Spongia sp. [49] |
Antioxidant effects | ROS generation, cell death | Natural products | Plant extracts [35] |
Estrogenicity | GFP induction in tg(cyp19a1b-GFP) | Environmental toxicology | Sediment extracts [52] |
vtg1 gene expession by qPCR | Environmental toxicology | Oil sand process waters [56] | |
Anti-convulsant | Locomotor activity, electrographic activity and epileptiform discharges | Natural products | Plant extracts [39] |
Inhibition of pentylenetetrazol-induced seizure activity, WISH for brain c-fos expression | Natural products | Plant extracts [34] | |
Fear behaviour | Alarm response, olfactory bulb activation in Tα1:GCaMP2 | Behavioural sciences | Skin extracts [59] |