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Table 2 Exposure setup for studies on natural products or environmental toxicology

From: The value of zebrafish as an integrative model in effect-directed analysis - a review

Exposure format Fish per well or vessel ( n ) Natural products Environmental toxicology
Medium volume per fish and respective reference Medium volume per fish and respective reference
96-well plates 1 100 μL [19,30,34,39,42] 100 μL [50]
4 <100 μL [45] -
24-well plates 1 - 2 mL [32]
5 n.a. [46] 200 μL [51,54,55]
10 100 to 250 μL [38,40,43] -
15 <100 μL [41] -
25 <100 μL [35] -
12-well plates 10 n.a. [48,49] -
6-well plates 25 to 30 n.a. [36,37] -
Beakers, dishes, vials 5 to 40 40 mLb [47] 0.45 to 5 mLa [53,56-58], 300 to 1,000 mLb [33,59]
  1. n.a. means information not available; abioassay with embryos and larvae, bbioassays with adults.