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Table 1 Survival rates (SR) of zebrafish ( Danio rerio ) eggs after vehicle injection

From: Microinjection into zebrafish embryos (Danio rerio) - a useful tool in aquatic toxicity testing?

Substance

Eggs

Replicates

Volume [nL]

24 h SR [%]

SD

96 h SR [%]

SD

Untreated

718

6

-

100

0

98

3.6

Autoclaved water

121

3

0.5

100

0

95

0

155

3

4.2

84

2.9

84

2.9

DMSO

158

3

0.5

88

5.9

88

8.7

90

3

4.2

60

5.0

55

6.2

Methanol

125

3

0.5

97

0.9

96

1.6

92

4

4.2

63

10.8

59

9.9

Triolein

94

3

0.5

97

2.4

96

0.4

110

3

4.2

94

3.5

94

3.5

  1. Untreated (pooled data) and after the pure injection of autoclaved tap water, DMSO, methanol, and triolein 24 and 96 h post fertilization (hpf), given with standard deviation (SD), respectively, total number of injected eggs, number of replicates, and injection volumes.