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Table 3 Regenerative results for G. tigrina exposed to different concentrations of the commercial disperse red 1

From: Effects of a textile azo dye on mortality, regeneration, and reproductive performance of the planarian, Girardia tigrina

Exposure time (h) Planarians/regeneration steps (mg/L)
Control 10 50 100 150 200
12 20a (a)b 20(a) 20(a) 20(a) 20(a) 20(a)
24 20(b) 20(b) 20(b) 20(b) 20(b) 3(t) 17(b)
48 20(c) 20(c) 20(c) 20(c) 20(c) 3(t) 17(c)
72 20(de) 20(de) 20(de) 2(c) 18(de) 2(c) 18(de) 3(t) 17(de)
96 20(f) 20(f) 3(d) 17(f) 5(d) 15(f) 2(d) 18(f) 3(t) 9(d) 8(f)
120 1(f) 19(g) 20(g) 2(f) 18(g) 5(f) 15(g) 5(f) 15 (g) 8 dead 3(t) 3(z) 4(d) 2(g)
Pdc120h   −1 1 4 4 9
  1. aNumber of animals. bRegeneration steps: (a) decapitated bodies, (b) beginning of blastema formation, (c) regular blastema, (d) beginning of auricle formation, (e) beginning of eyespots formation, (f) auricle and eyespots clearly defined, (g) full head formation, (t) body section without cicatrization, (z) without body cicatrization, with eyespots, and without auricle. cPd is the number of planarians with delayed regeneration subtracted from the number of delayed planarians in the control, Pd greater or equal to 1 is considered as adverse, for more information see ‘Methods’ section.