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Table 2 Endpoints of the fish embryo test with the zebrafish (based on Nagel[22])

From: Embryotoxic and proteotoxic effects of water and sediment from the Neckar River (Southern Germany) to zebrafish (Danio rerio) embryos

Observed endpoints

Hours post-fertilization

8

12

24

48

60

72

84

96

Mortality

Hatching

    

Slowed ontogenesis/Retardation

        

  Epiboly

       

  Gastrulation

 

      

  Exogastrulation

 

      

  Formation of somites

  

     

  Tail detachment

  

     

  Spontaneous movements

  

     

  Development of eyes

  

     

  Number of heartbeats (beats/min)

   

    

  Blood circulation

   

    

  Sacculi/Otolith formation

    

   

  Presence of melanocytes

    

   

Developmental failures

        

  Oedema

    

  Malformation of head

    

  Tail deformation

     

  Pigmentation failure

     

  Helical body

     

  Scoliosis