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Table 1 Mean hatching times during the zebrafish embryo test

From: Embryotoxic and proteotoxic effects of water and sediment from the Neckar River (Southern Germany) to zebrafish (Danio rerio) embryos

Mean hatching time

Negative control

Site 1

Site 2

Site 3

Site 4

May 2011

66 hpf

69 hpf

n.a.

58 hpf

79 hpf

September 2011

67 hpf

63 hpf

n.a.

69 hpf

87 hpf

May 2012

72 hpf

81 hpf

93 hpf

81 hpf

85 hpf

September 2012

68 hpf

59 hpf

26% at 96 hpf

63 hpf

68 hpf

  1. Mean hatching time is defined as the time point when 50% of the surviving fish embryos hatched. Mean hatching times were calculated in SigmaPlot 10.0 by graphic analysis of the combined hatching curves of two independent replicates. n.a. not available.