Indication level | Test |
---|---|
Toxic potentials | umu-test, Ames test (genotoxicity) |
in vitro reporter gene assays (vertebrate cells, yeasts) controlled by Ah-receptor (dioxin-like toxicity) | |
GH3, RTL-W1-cell culture (cytotoxicity) | |
ELS-tests with zebrafish (developmental toxicity) | |
Growth inhibition test with Lumbriculus variegatus (developmental toxicity) | |
Growth inhibition test with Lemna minor (phytotoxicity) | |
Toxic effects | Early life stage tests with trout (developmental toxicity) |
Acetylcholinesterase inhibition in the fish brain (neurotoxicity) | |
Cytochrome P450IA1 (EROD) in fish liver and gills (dioxin-like toxicity) | |
Histopathology of fish liver, gills, and kidney and gammarid tissues (cytotoxicity, fish and invertebrate health) | |
Stress protein Hsp 70 (proteotoxicity) | |
Micronucleus test in fish blood cells (genotoxicity) | |
Macrozoobenthos community (community integrity) | |
Endocrine potentials | E-Screen (estrogenicity) |
Reporter gene assays in vitro (estrogenicity, androgenicity, anti-androgenicity) | |
Reproduction test with the snail Potamopyrgus variegatus (estrogenicity) | |
Endocrine effects | Vitellogenin in juvenile and male trout (estrogenicity) |
Gonad histology of fish and gammarids, | |
Gonadosomatic index (GSI) in fish (estrogenicity, androgenicity) | |
Sex ratio and fecundity in gammarids (estrogenicity, androgenicity) |