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Table 4 Effects of TiO 2 (P25) in the earthworm reproduction test

From: Influence of application techniques on the ecotoxicological effects of nanomaterials in soil

Test concentration [mg/kg]1

Mortality [%]

Mean biomass per vessel at test start ± SD2[g]1

Biomass per vessel at test end ± SD2[g]1

Increase in biomass of adult worms after 28 d [%]3

Number of juveniles per test vessel ± SD2

0 (control)

0

3.29 ± 0.24

5.47 ± 0.36

67

212 ± 46

Application via powder on soil

50

0

3.37 ± 0.43

5.80 ± 0.15

74

295 ± 44 *

100

0

3.49 ± 0.13

5.47 ± 0.10

57

299 ± 74

200

0

3.45 ± 0.14

5.47 ± 0.11

59

315 ± 42

Application via powder on food

50

0

3.36 ± 0.25

5.48 ± 0.32

63

280 ± 84

100

0

3.17 ± 0.26

5.41 ± 0.33

71

309 ± 67 *

200

0

3.29 ± 0.27

5.37 ± 0.33

63

333 ± 71 *

Application via dispersion on soil

10

0

3.25 ± 0.12

5.15 ± 0.12

58 *

326 ± 74 *

20

0

3.33 ± 0.25

5.69 ± 0.15

71

321 ± 14 *

Application via dispersion on food

10

0

3.23 ± 0.09

5.04 ± 0.10

56 *

280 ± 52

20

0

3.21 ± 0.16

5.26 ± 0.13

64

279 ± 12

  1. 1 All concentrations refer to soil dry mass. 2 SD: standard deviation.3 Values indicate the increase in biomass. Asterisks indicate a statistically significant difference to control (significance α = 0.05; one-sided smaller, Williams multiple sequential t-test).