Table 6 OECDs reporting requirements, modified evaluation criteria/questions concerning test environment, and summary of the evaluation results.
Evaluation method | Evaluation criteria/question | Type of criteria/mark | Summary of evaluation results |
|---|---|---|---|
Klimisch et al. | Data on physical and chemical test conditions (pH, conductivity, light intensity, temperature, hardness of water). | Recommended | Temperature is not stated in two studies, pH is not stated in seven studies, conductivity is not stated in any of these studies, hardness of water is not stated in any of these studies, light intensity is not stated in two studies. |
Data on the feeding of the test animals (chronic studies). | Recommended | Stated in all studies. | |
Durda and Preziosi | Water characteristics recorded (e.g., pH, DO, temperature). | Recommended | Temperature is not stated in two studies, pH is not stated in seven studies, DO is not stated in three studies. |
Light intensity and/or photoperiod. | Recommended | Not stated in two studies. | |
Physical structure of test environment recorded. | Recommended | Not stated in one study. | |
Number of animals per test apparatus (e.g., aquaria) noted. | Recommended | Not stated in one study. | |
Feeding protocols noted (long-term tests). | Mandatory | Not stated in one study. | |
Food composition noted/known. | Recommended | Not stated in one study. | |
Hobbs et al. | Was the temperature measured and stated (3 marks)? Award 1 mark if only the temperature settings of the room or chamber are stated? | 0, 1, or 3 | Not stated in two studies and only specified in two studies. |
For tests not using aquatic macrophytes and alga (i.e., non-plant), was the dissolved oxygen content of the test water measured during the test? | 0 or 3 | Not stated in three out of five studies. | |
Was the pH measured and values stated? Award 1 mark if it is measured but not stated or if the pH of the dilution water only is measured and stated. | 0, 1, or 3 | Not stated in seven studies. | |
For marine and estuarine water, was the salinity/conductivity measured and stated? | 0 or 3 | No marine studies were evaluated. | |
Was the type of test media used stated? | 0 or 5 | Not stated in three studies. | |
Schneider et al. | For repeated dose toxicity studies only: Is information given on the housing or feeding conditions? | Recommended, 0, or 1 | No repeated dose toxicity studies were evaluated. |
OECD guidelines | Photoperiod, light intensity and quality (source, homogeneity); pH values at the beginning and at the end of the test at all treatments; hardness; temperature; dissolved oxygen concentration; test vessel description including volume; detailed information on feeding (e.g., type of food(s), source, amount given and frequency); residual chlorine levels (if measured); TOC and COD; Salinity of the test medium. | Mandatory | The non-standard studies were not evaluated according to the OECD reporting requirements. |