Regrettable substitution? Comparative study of the effect profile of bisphenol A and eleven analogues in an in vitro test battery

Environmental Sciences Europe

Table 3 EC₅₀ values in the Yeast Anti-Androgen Screen (YAAS)

YAAS	BPA	BPB	BPE	BPF	BPZ	BPAF	BPAP	BPBP	BADGE	TBBPA	TCBPA
EC₅₀ (mg/L)	1.06	0.854	0.232	0.304	2.08	0.916	1.68	0.785	2.21	0.139	0.155
95% confidence interval (mg/L)	0.959–1.16	0.762–0.946	0.210–0.254	0.260–0.348	1.84–2.32	0.805–1.03	1.48–1.87	0.661–0.909	1.96–2.48	0.118–0.161	0.130–0.180
Specific activity (M)	4.63 × 10⁻⁶	3.52 × 10⁻⁶	1.08 × 10⁻⁶	1.52 × 10⁻⁶	7.75 × 10⁻⁶	2.72 × 10⁻⁶	5.79 × 10⁻⁶	2.23 × 10⁻⁶	6.49 × 10⁻⁶	2.56 × 10⁻⁷	4.23 × 10⁻⁷
Maximum relative activity (%)	111.0	112.2	100.0	111.8	104.1	113.5	111.7	109.9	107.6	115.6	138.2
Significance to BPA	–	↓	↓	↓	↑	ns	↑	↓	↑	↓	↓

Displayed are the 50% effect concentrations (EC₅₀) (mg/L) with the respective 95% confidence interval (mg/L), specific activity (M), maximum anti-androgenic induction (%), and the significant difference to BPA for each bisphenol. The specific activity is calculated using Eq. 1. The significant difference to BPA is determined through non-overlapping confidence intervals. Bold arrows pointing downwards indicate a significantly lower EC₅₀ than BPA, arrows pointing upwards indicate a significantly higher EC₅₀. ns = not significant. Bisphenols with no values did not show an effect at the tested concentrations