Regrettable substitution? Comparative study of the effect profile of bisphenol A and eleven analogues in an in vitro test battery

Environmental Sciences Europe

Table 1 EC₅₀ values in the Yeast Estrogen Screen (YES)

YES	BPA	BPB	BPE	BPF	BPS	BPZ	BPAF	BPAP	TCBPA
EC₅₀ (mg/L)	0.516	0.407	0.500	0.509	33.2	0.120	0.146	3.05	0.612
95% confidence interval (mg/L)	0.504–0.528	0.379–0.435	0.482–0.517	0.485–0.533	14.5–17.6	0.115–0.125	0.142–0.151	2.62–2.79	0.568–0.602
Specific activity (M)	2.26 × 10⁻⁶	1.68 × 10⁻⁶	2.33 × 10⁻⁶	2.54 × 10⁻⁶	6.42 × 10⁻⁵	4.47 × 10⁻⁷	4.34 × 10⁻⁷	9.32 × 10⁻⁶	1.60 × 10⁻⁶
Maximum relative activity (%)	94.2	92.3	97.8	105.2	79.6	85.2	82.1	106.5	110.9
Significance to BPA	–	↓	ns	ns	↑	↓	↓	↑	↑

Displayed are the 50% effect concentrations (EC₅₀) (mg/L) with the respective 95% confidence interval (mg/L), specific activity (M), maximum estrogenic induction (%), and the significant difference to BPA for each bisphenol. The specific activity is calculated using Eq. 1. The significant difference to BPA is determined through non-overlapping confidence intervals. Bold arrows pointing downwards indicate a significantly lower EC₅₀ than BPA, arrows pointing upwards indicate a significantly higher EC₅₀. ns = not significant. Bisphenols with no values did not show an effect at the tested concentrations