Table 6 Assessment of plant response to abiotic stress under containment in GMP applications (numbers of studies in brackets)
Plant | Potato (2) | Maize (4) | Maize (1) |
|---|---|---|---|
Containment | Climate chamber | Growth chamber (2), greenhouse (2) | Greenhouse |
Abiotic stress assessed | Cold stress | Cold, heat, drought, salt stress | Drought stress |
Artificially induced stress | Yes | Yes | Yes |
Experimental design | Tubers placed on soil surface and tubers covered with soil (10Â cm and 20Â cm depth) | Plants grown in pots (V3 or V4 growth stage) | Plants grown in pots (V4/V5 growth stage) |
Test regimes | 2 experiments with low starting temperatures, gradually lowering to minimum temperatures followed by gradual thawing | 3 stress treatments (mild, moderate, severe) | 1 drought cycle |
Parameters assessed | Survival rate of tubers (developing sprouts were assessed after 2–3 weeks at 18 ℃) | Growth and development parameters: plant height, growth stage, chlorophyll content, plant vigour, fresh weight, dry weight of above-ground biomass, necrosis (heat), leaf rolling (drought), electrical conductivity (salt) | Physiological parameters: photosynthesis, stomatal conductance, leaf extension rate (LER), ion leakage and relative water content (RWC) |
Duration of stress in days | 8–12 | Cold: 8, Heat: 5, Drought: 15, Salt: 12 | 6 |