Table 3 Pollen viability assessments in GMP applications (number of studies in brackets)
| Â | Oilseed rape (1) | Soybean (1) | Maize (5) |
|---|---|---|---|
Plants derived from | Growth chamber | Field trial | Field trial |
Environmental conditions | 21°/18 ℃ (day/night), 16 h photoperiod | n.i | Well-watered and water-limited conditions |
Purpose of the study | Assessment of pollen viability and morphology | Assessment of pollen viability and morphology | Assessment of pollen viability and morphology |
Parameters assessed | Pollen grain diameter, % viable pollen, morphology | Pollen grain diameter, % viable pollen, morphology | Pollen grain diameter, % viable/non-viable pollen, general morphology |
Methodology | Alexander’s stain, micrographs | Alexander’s stain | Alexander’s stain, Lugol staining, microscopic examination |