Table 3 Two-way ANOVA of the effects of primer pairs and DNA polymerases on fish richness detection in surface water and sediment samples
From: Choice of primer pairs and PCR polymerase affect the detection of fish eDNA
Factors | SS | df | MS | F (DFn, DFd) | P value |
|---|---|---|---|---|---|
Water samples | |||||
 Primer pairs | 18,548 | 4 | 4637 | F (1.502,3.004) = 1233 | < 0.0001 |
 DNA polymerases | 1115 | 2 | 557.3 | F (1.006,2.012) = 122.0 | 0.0079 |
 Primer * polymerases | 793.7 | 8 | 99.21 | F (1.734,3469) = 12.38 | 0.0272 |
Sediment samples | |||||
 Primer pairs | 17,966 | 4 | 4429 | F (1.271,2.542) = 220.4 | 0.0015 |
 DNA polymerases | 3819 | 2 | 191.0 | F (1.022,2.004) = 60.51 | 0.0160 |
 Primer * polymerases | 1706 | 8 | 213.2 | F (1800,3600) = 29.18 | 0.0062 |