Table 2 The amplification speed and advantages of three commercial DNA polymerases, and all DNA polymerases are premixes
From: Choice of primer pairs and PCR polymerase affect the detection of fish eDNA
Polymerase/mix | Amplification speed | Advantages |
|---|---|---|
2 × Taq plus Master Mix II (Dye Plus) | 60 s/kb | Ready-to-use premixes, easy to handle |
2 × Rapid Taq Master Mix | 15 s/kb | High amplification performance, high specificity, high stability and high yield |
2 × Phanta Flash Master Mix (Dye Plus) | 4–5 s/kb | Broad template compatibility, excellent amplification speed and yield, stable crude amplification, perfect high GC suitability, ultra-high fidelity |