Table 1 Summary of five primer pairs in the mitochondrial 12s rRNA gene region used for fish eDNA sequencing in the current study, including primer name and sequences, annealing temperature (Ta), amplicon size (bp) and original references
From: Choice of primer pairs and PCR polymerase affect the detection of fish eDNA
Primer pairs | Primer sequence (5ʹ-3ʹ) | Ta/℃ | Size/bp | References |
|---|---|---|---|---|
Mifish-E | Forward: GTTGGTAAATCTCGTGCCAGC Reverse: CATAGTGGGGTATCTAATCCTAGTTTG | 53 | 171 | [37] |
Riaz-12S | Forward: ACTGGGATTAGATACCCC Reverse: TAGAACAGGCTCCTCTAG | 55 | 106 | [42] |
Teleo | Forward: ACACCGCCCGTCACTCT Reverse: CTTCCGGTACACTTACCATG | 52 | 100 | [60] |
Tele02 | Forward: AAACTCGTGCCAGCCACC Reverse: GGGTATCTAATCCCAGTTTG | 56.8 | 167 | [57] |
12S-V5 | Forward: TAGAACAGGCTCCTCTAG Reverse: TTAGATACCCCACTATGC | 55 | 106 | [42] |