Fig. 3
From: Choice of primer pairs and PCR polymerase affect the detection of fish eDNA
Distribution of taxonomic classification levels of fish (a), the composition of taxa at the order level (b), and fish taxonomic richness of all 15 groups of primer pairs and DNA polymerases in surface water samples (c). ‘Unknown’ indicates reads not assigned to known taxonomic taxa, and the “a”, “b” and “c” in panel C are the significance test by the one-way ANOVA, respectively