Fig. 1
From: Chronic toxicity testing including transcriptomics-based molecular profiling in Cloeon dipterum
Schematic representation of the long- and short-term toxicity test workflow with C. dipterum. Long-term toxicity test: field-collected young larvae in the larval stage L3 were exposed to a control condition and five test concentrations containing 38.0–600 ng/L fipronil in Elendt M4 medium. For each treatment, 4 replicates containing 5 larvae each were prepared. The test design was semi-static with two media renewals per week. Larvae were fed with diatoms of N. pelliculosa and carrots. The apical endpoints larval development, emergence and mortality were determined twice per week. The test was conducted for 38 days. Short-term exposure test: The test was conducted for a total test duration of 7 days. Three larvae per replicate and condition were used for RNA extraction, RNA sequencing-based transcriptomics and molecular profiling. (Created with BioRender.com)