Table 2 Assay for microcystin-ADDA-ELISA
Microcystin-ADDA ELISA | |||||||||
|---|---|---|---|---|---|---|---|---|---|
OD at 450 nm | |||||||||
Sample | Concentration (µg/L) | Singlet | Duplicate | Triplicate | Mean | %B/B0 | Microcystin (µg/L) | %B/B0 | |
Standard | 0 | 1.745 | 1.762 | 1.798 | 1.768333333 | 100 | 0 | 100 | |
0.15 | 1.64 | 1.639 | 1.633 | 1.637333333 | 92.5918944 | 0.15 | 92.5918944 | ||
0.4 | 1.494 | 1.364 | 1.377 | 1.411666667 | 79.8303487 | 0.4 | 79.8303487 | ||
1 | 1.044 | 1.027 | 1.062 | 1.044333333 | 59.0574929 | 1 | 59.0574929 | ||
2 | 0.681 | 0.649 | 0.687 | 0.6723333333 | 38.0207352 | 2 | 38.0207352 | ||
5 | 0.59 | 0.56 | 0.61 | 0.5866666667 | 33.1762488 | 5 | 33.1762488 | ||
Sample | Concentration (µg/L) | Singlet | Duplicate | Triplicate | Mean | %B/B0 | Microcystin (µg/L) | ||
|---|---|---|---|---|---|---|---|---|---|
Leptolyngbya | 100 | 0.092 | 0.996 | 1.101 | 0.096333 | 5.44769086 | 17.72 | ||
Desertifilum | 100 | 0.736 | 0.069 | 0.072 | 0.067667 | 3.8265787 | 19.38 | ||
Singlet | Duplicate | Triplicate | Mean | ||||||
Positive control | 0.75 (ppb) | 1.163 | 1.146 | 1.152 | 1.153666667 | 65.2403393 |