| RBT | Simulation test (for half-life determination) |
---|---|---|
Test temperature | 20–25 ℃ | 12 ℃ |
Inoculum source | Activated sludge/sewage effluent/mixture of soil and surface water | Environmental samples of soil, sediment, or water |
Inoculum concentration | 104–108 cells/l | Highly variable (based on environmental sample) |
Test substance concentration | 2 to > 100 mg/l |  < 1–10 μg/l (for OECD 309) |
Test substance metabolism | Sole carbon source | Not the primary substrate, allowing for competing substrates and co-metabolism |
Kinetics | Growth linked | First order or biphasic |