Table 1 List of primer used for qPCR
Primer name | Sequence (5′–3′) | Target genes | qPCR efficiency (%) | Amplification factor |
|---|---|---|---|---|
MTC_forward MTC_reverse | AGCCAATATTTTCGAGTGGAGA CAAGATGCTCGAATAGCAACAGTA | Metallothionein-like containing protein [20] | 87.50 | 1.87 |
GST_forward GST_reverse | CATCAACATTGCCGACAAAC ACGACAGCCTTGAACTTTGC | Glutathione S-transferase [22] | 96.53 | 1.97 |
YWHAZ_forward YWHAZ_reverse | TCGCCCTCAACTTTTCCGTT TGCTATCGCTTCATCGAATGC | Tyrosine 3-monooxygenase [23] | 88.44 | 1.88 |