Efficiency of advanced wastewater treatment technologies for the reduction of hormonal activity in effluents and connected surface water bodies by means of vitellogenin analyses in rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta f. fario)

Environmental Sciences Europe

Table 5 Time schedule for the exposure experiments performed at WWTPs and bypass stations with 1-year-old trout

Start of exposure	End of exposure	Exposure duration (days)	Exposure type	Trout species
Winter season 2012/2013 prior to the upgrade
15 Nov 2012	24 Jan 2013	70	Laboratory neg. control + EE₂ control	Brown and rainbow trout
15 Nov 2012	17 Jan 2013	63	Cage exposure	Rainbow trout
15 Nov 2012	14 Feb 2013	91	Exposure in bypass systems	Brown and rainbow trout
6 Feb 2013	21 Mar 2013	43	Exposure at WWTP Eriskirch	Rainbow trout
Winter season 2013/2014 after the upgrade
	29 Jan 2014	0	Neg. control from hatchery	Brown and rainbow trout
2 Dec 2013	23 Jan 2014	52	EE₂ control	Brown and rainbow trout
2 Dec 2013	4 Feb 2014	64	Cage exposure	Rainbow trout
2 Dec 2013	13 Feb 2014	73	Exposure at WWTP Eriskirch	Rainbow trout
2 Dec 2013	12 Mar 2014	100	Exposure in bypass systems	Brown and rainbow trout