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Environmental Sciences Europe
Fig. 7 | Environmental Sciences Europe

Fig. 7

From: Efficiency of advanced wastewater treatment technologies for the reduction of hormonal activity in effluents and connected surface water bodies by means of vitellogenin analyses in rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta f. fario)

Fig. 7

a Absorbance measured in blood plasma samples of 1-year-old brown trout exposed at the bypass stations in 2013 and 2014; means and SD are shown. All samples of a group were analyzed within one semi-quantitative vitellogenin salmonid (Salmoniformes) biomarker ELISA kit (enzyme activity = color intensity is proportional to the concentration of vitellogenin in the sample). N-numbers 2013 females: negative control n = 4, Argen n = 4, Schussen n = 3; males: negative control n = 0, Argen n = 4, Schussen n = 4. No significant differences; p > 0.05. N-numbers 2014 females: negative control n = 6, Argen n = 6, Schussen n = 9; males: negative control n = 10, Argen n = 13, Schussen n = 5. Significant differences with the Tukey–Kramer HSD test: females 2014 neg. control—Schussen p = 0.0231 (Asterisks significant differences; *p < 0.05). b Values of a relative to negative control. Neg. control was set to 100 %. In 2013, no values could be given for males because of absence of males in the neg. control

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