The value of zebrafish as an integrative model in effect-directed analysis - a review

Environmental Sciences Europe

Table 2 Exposure setup for studies on natural products or environmental toxicology

Exposure format	Fish per well or vessel ( n )	Natural products	Environmental toxicology
Exposure format	Fish per well or vessel ( n )	Medium volume per fish and respective reference	Medium volume per fish and respective reference
96-well plates	1	100 μL [19,30,34,39,42]	100 μL [50]
96-well plates	4	<100 μL [45]	-
24-well plates	1	-	2 mL [32]
	5	n.a. [46]	200 μL [51,54,55]
	10	100 to 250 μL [38,40,43]	-
	15	<100 μL [41]	-
	25	<100 μL [35]	-
12-well plates	10	n.a. [48,49]	-
6-well plates	25 to 30	n.a. [36,37]	-
Beakers, dishes, vials	5 to 40	40 mL^b [47]	0.45 to 5 mL^a [53,56-58], 300 to 1,000 mL^b [33,59]

n.a. means information not available; ^abioassay with embryos and larvae, ^bbioassays with adults.